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. 2001 Apr;21(7):2533–2544. doi: 10.1128/MCB.21.7.2533-2544.2001

TABLE 2.

Activation of the dpy-7::GFP reporter gene following EMS blastomere isolation in several independent transgenic lines containing chromosomally integrated hs-elt-3 or hs-elt-1 constructsa

Strain Mean no. of dpy-7::GFP-positive nuclei from isolated EMS (SD) [no. of embryos examined]
hs-elt-3
 JM58 4.5 (2.9) [6]
 JM59 6.1 (2.1) [19]
 JM60 8.3 (3.5) [11]
hs-elt-1
 JM54 13.8 (4.6) [9]
 JM55 15.7 (3.9) [10]
a

One hour after EMS isolation, embryos were heat shocked at 33.5°C for 40 min and then allowed to recover for 16 to 20 h. For each strain, control embryos were allowed to develop following laser ablation without the application of heat shock; none of these control embryos produced dpy-7::GFP-positive nuclei (data not shown).