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. 2021 Dec 20;7:397. doi: 10.1038/s41420-021-00797-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Young and aged BMDMs derived from mouse bone marrow were infected with Ad-mCherry-GFP-LC3 (20 MOI) on the 5th day when the cells were not fully mature and then treated with LPS (100 ng/ml) on the 7th day. A Representative laser confocal microscopy images. B Transmission electron microscopy (TEM) examination of autophagosomes from young and aged BMDMs and (C) quantification. D Immunoblotting of LC3-I/II, ATG5 and p62 expression in BMDMs from each group. Quantification in (E). F Representative IF staining of LC3-I/II and ATG5 incorporation (red fluorescence) with DAPI counterstaining (blue fluorescence) in BMDMs. Quantification in (G). All results are representative of at least three independent experiments. Values are presented as the mean ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.