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. 2021 Dec 20;7:397. doi: 10.1038/s41420-021-00797-2

Fig. 6. Autophagy alters macrophage polarization in aged and young mice.

Fig. 6

Young and aged BMDMs were treated with LPS (100 ng/ml) with or without pretreatment with 3-MA (5 mM) or LAC-ATG5. A Representative IF staining of CD206 and iNOS incorporation (red fluorescence) with DAPI counterstaining (blue fluorescence) in BMDMs from young and aged BMDMs after 3-MA pretreatment. Quantification in (B). C Representative IF staining of CD206 and iNOS incorporation (red fluorescence) with DAPI counterstaining (blue fluorescence) in BMDMs from each group after pretreatment with LAC-ATG5. Quantification in (D). E Immunoblotting of STAT1, P-STAT1, STAT6, and P-STAT6 expression in young and aged BMDMs was performed after 3-MA pretreatment. Quantification in (F). G Immunoblotting of STAT1, P-STAT1, STAT6, and P-STAT6 expression in BMDMs from each group was performed after pretreatment with LAC-ATG5. Quantification in (H). All results are representative of at least three independent experiments. Values are presented as the mean ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.