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. 2021 Dec 20;13(1):18. doi: 10.1038/s41419-021-04467-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B KG1a and MOLM-13 cells transfected with WTIP vector were treated with doxycycline for the indicated times, expression of FOXO3a protein and mRNA were detected by western blot analysis and RT-PCR, respectively. Data are represented as mean ± SD from three independent experiments (***P < 0.001). C Western blot analysis of the indicated proteins in KG1a and MOLM-13 cells transfected with shWTIP or shCtrl (control vector). D Western blot analysis of FOXO3a and PUMA expression in WTIP-overexpressing KG1a and MOLM-13 cells transfected with FOXO3a siRNAs (siFOXO3a-1) or negative control siRNA (siNC). E Flow-cytometric analysis of apoptosis in KG1a and MOLM-13 cells transfected with FOXO3a siRNAs (siFOXO3a-1) or negative control siRNA (siNC), as determined by using Annexin V-FITC and propidium iodide (PI). Data are represented as mean ± SD from three independent experiments (**P < 0.01).