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. 2021 Dec 20;13(1):18. doi: 10.1038/s41419-021-04467-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A MOLM-13 cells transfected with WTIP or control vector were treated with doxycycline for 48 h, and cell lysates were subjected to immunoprecipitation and immunoblotting with anti-WTIP and anti-FOXO3a antibodies, respectively. B FOXO3a-Luc reporter vector was transfected into HEK293 cells, either in the presence of WTIP or control vector, and luciferase activity was measured 48 h after transfection. Data are represented as mean ± SD from three independent experiments (*P < 0.05). C Confocal immunofluorescence analysis of FOXO3a localization in KG1a and MOLM-13 cells transfected with WTIP or control vector. Scale bars, 50 μm.