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. 2021 Dec 20;7:398. doi: 10.1038/s41420-021-00795-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A, B Overexpression of UBQLN4 by transfecting a plasmid in both HCT-8 and SW480 cells. The efficiency was detected using RT-qPCR (A) and western blot (B). The MTS experiment indicated that UBQLN4 overexpression promoted the growth of both HCT-8 (C) and SW480 cells (D). The plate colony formation experiment was also utilized to detect the effect of UBQLN4 overexpression on the proliferation of HCT-8 (E) and SW480 cells (F). Transwell experiment of the migration and invasion of UBQLN4-overexpressing HCT-8 (G) and SW480 cells (H). 200×, Scale bar: 200 μm. Wound healing experiment showing the migration of UBQLN4-overexpressing HCT-8 (I) and SW480 cells (J). 100×, Scale bar: 200 μm. Schematic of the CytoSelect™ 24-Well Cell Migration Assay (K) and respective quantification of HCT-8 or SW480 cell migration and invasion after the upregulation of UBQLN4 (L). All data from at least three independent experiments are presented as the means ± SD. *p < 0.05, **p < 0.01, *** or ****p < 0.001.