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. 2021 Dec 7;12:791753. doi: 10.3389/fimmu.2021.791753

Figure 1.

Figure 1

Effect of N-protein on lung inflammation. (A) His-tagged SARS-CoV-2 N-protein was purified on a Ni-NTA column. Samples at various stage of purification were run on 12% SDS-PAGE gel and stained with Coomassie blue. M: molecular weight marker. LPS removal: LPS removed via a commercial kit after purification. (B) Purified N-protein was separated on 12% SDS-PAGE gel and examined for N-protein identity via Western blot analysis. M: molecular weight marker. (C) N-protein was pretreated with polymyxin B (250 µg/ml) for 1 h at 37°C. C57BL/6 mice (6-8 weeks old) were treated with PBS or N-protein (75 µg/mouse) intratracheally. Lung samples were harvested at 24 h after N-protein insult. Tissue sections were stained with H&E and viewed at x100 magnification. Leukocyte infiltration was scored semi-quantitatively from 0 (normal tissue) to 3 (intensive filtration) from 6 randomly selected fields in three tissue sections per mouse from four mice. Data are presented as mean ± SD, n = 4. **p < 0.01.