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. 2021 Dec 7;12:791753. doi: 10.3389/fimmu.2021.791753

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Copyright © 2021 Xia, Tang, Wang, Lai, Xu, Huang, Hu, Gong, Fan, Shu and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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M1 polarization of alveolar macrophages after N-protein treatment in vivo. C57BL/6 mice were treated with PBS or N-protein (75 µg/mouse in 50 µl) intratracheally. Total cells in the BAL were harvested at 24 h and counted using a hemocytometer. (A) Alveolar macrophages in the BAL were identified as F4/80+ in flow cytometry and quantitated by the percentage of the positive cell population and the total cells in the BAL. Dot plots showed the gating scheme for determining the frequency of F4/80+CD86+ (M1) (B), F4/80+iNOS+ (M1) (B), and F4/80+CD206+ (M2) (C) cells in flow cytometry. The numbers in the dot plots represent the percent of the population within the specific gate. Percentages of the M1/M2 populations were summarized in the panels at right. Data are presented as mean ± SD, n = 6-8. ***p < 0.001.