Table 3.
Comparison of agglutinative activity of two bacteria-binding hemocyanin and deglycosylation hemocyanin with 75 μg/ml.
| HMC-Vp | dN-HMC-Vp | dO-HMC-Vp | HMC-Vf | dN-HMC-Vf | dO-HMC-Vf | |
|---|---|---|---|---|---|---|
| Agglutinative titer a /Agglutinative specific activity b | ||||||
| S. agalactiae | 512/0.15 | 256/0.29 | 2/37.5 | 64/1.17 | 64/1.17 | 1/75 |
| V. parahaemolyticus | 512/0.15 | 256/0.29 | 1/75 | 128/0.59 | 64/1.17 | 1/75 |
| V. alginolyticus | 256/0.29 | 256/0.29 | 1/75 | 64/1.17 | 64/1.17 | 1/75 |
| V. fluvialis | 128/0.59 | 128/0.59 | 2/37.5 | 256/0.29 | 128/0.59 | 2/37.5 |
| E. coli K12 | 256/0.29 | 128/0.59 | 2/37.5 | 128/0.59 | 128/0.59 | 1/75 |
a
The highest dilution of the testing samples in the presence of different bacteria.
b
Agglutinative activity (μg/ml) = Protein concentration (μg/ml)/agglutinative titer.