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. 2021 Oct 7;13(2):583–597. doi: 10.1016/j.jcmgh.2021.09.013

Figure 3.

Figure 3

TMEM199 Ala7Glu mouse model on chow diet. Liver and plasma of 8-week-old mice were collected in liquid nitrogen or heparin coated tubes, respectively, after 4-hour fast. (A) QPCR and Western blot for hepatic TMEM199 mRNA (left) and protein expression (right). (B) Body weight and liver weight to body weight ratio; liver to body weight ratio was significantly increased. (C) Biochemical plasma lipid panel; reduced HDL and increased plasma TG were observed. (D) TMEM199-Ala7Glu mice have increased hepatic TG (left) and visible hepatic steatosis (right). (E and F) mRNA expression for genes involved in lipid metabolism in mouse livers. (G) Plasma of control and heterozygous Tmem199-Ala7Glu mice were pooled for FPLC analysis (n = 5 per group; littermates), and fractions were analyzed for TG and cholesterol content. (H) ApoB100/48, PCSK9, and apoA1 were analyzed from 7 μL whole plasma on Western blot. (I and J) MTTP and SREBP2, respectively, on Western blots of mouse liver lysates. All panels show representative Western blots from 3 experiments with triplicate measurements per experiment. ∗∗∗P < .001, ∗∗P < .01, ∗P < .05 as calculated with unpaired Mann-Whitney test. (K) N-glycan MALDI-TOF profiles derived from mouse plasma.