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. 2021 Nov 20;298(1):101441. doi: 10.1016/j.jbc.2021.101441

Figure 2.

Figure 2

Structural and functional conservation between human and zebrafish IMPDH1.A, negative stain EM of purified human retinal variants IMPDH1(546) & IMPDH1(595) and zebrafish Impdh1a (544) & Impdh1a_tvX1. The scale bar represents 100 nm. B and C, sequence alignment of human retinal variants IMPDH1(546) and IMPDH1(595), zebrafish Impdh1a (544) NM_001002177.1, and zebrafish retinal variant Impdh1a_tvX1 (XM_005159007.4). B, conserved N-terminal alpha-helix in blue and gene beginning in pink. C, residues in orange (ordered) are resolved in cryo-EM structures of the human protein (9), remainder of C-terminus in yellow is flexible and not resolved in structures (9). D, GTP-inhibition curves of zebrafish Impdh1a (544) (solid gray line), Impdh1a_tvX1 (solid black line), and nonassembly Y12A protein (dashed black line). Each data point represents a triplicate and the error bars are standard deviation. impdh, inosine monophosphate dehydrogenase; tv, transcript variant.