Table 1.
Primers
| Target/NCBI reference sequence | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| Gene amplification | ||
| impdh1a/NM_001002177.1 | ACAGAGAACAGATTGGTGGATCCATGGCTGATTACCTGATCAGCGG | GAGTGCGGCCGCTTAGCTGCTGTGTTTAGTGACGGC |
| impdh1b/NM_001014369.2 | ACAGAGAACAGATTGGTGGATCCATGGCAGACTATCTGATAAGCGGAGGAA | GAGTGCGGCCGCTTAGTAAAGACGCTTCTCGAAGGAGTGAAGTC |
| impdh2/NM_201464.1 | ACAGAGAACAGATTGGTGGATCCATGGCGGACTATTTAATCAGCGG | CTCGAGTGCGGCCGCTTAAAACAGGCGCTTCTCATAGCTGT |
| impdh1a_tvX1/XM_005159007.4 impdh1a_tvX2/XM_005159008.4 | TTTGGGGAAATCAGTCCAAC | GAAGCCCGAACAAAACAAAA |
| impdh1a_tvX3/XM_009293441.2 | GAAGCCCGAACAAAACAAAA | GGAGACCCTTCATCCACAGC |
| impdh1a_tvX4/MZ851785 | GGAGACCCTTCATCCACAGC | GTTGAGAAATGAGCGCCTCT |
| Genotyping | ||
| impdh1aˆsa23234 | GACGCCACTCATTTCCTCTC | CTTTCCGGACCTCGTTAGC |
| rhoˆfl9 | CCCTTCGAAGAGGAAGAGG | TCGAGAGTGTCTGGAAGGAGA |
| qPCR | ||
| impdh1a (all variants) | CATTGCAGGAATTCAACACG | GAACTCCACCCTCAACCTGA |
| impdh1b (all variants) Set 1 | AGCAAACTAGTCGGCATCGT | GGCAGGAGCGACTACAAGAT |
| impdh1b (all variants) Set 2 | ATCTGATAAGCGGAGGAACG | CCAGGCAAGATCAGGAAGTC |
| impdh2 (all variants) | TCTGGAGACGGCCTTACCTA | ATAGGCGACGAGATCAGTGG |
| impdh1a_tvX1 & X2 (N-term) | AATGGCCCGTTTACTCACAG | GTAATCAGCCATGCTCTCTGG |
| impdh1a_tvX3 & X4 (N-term) | GGCGAATATTCAGCAGAAGG | ATCACCGATGGCAAAGAGTT |
| impdh1a_tvX1 & X3 (C-term) | CATTGCAGGAATTCAACACG | TAAATGGCAAAAAAGAATATGA |
| impdh1a_tvX2 & X4 (C-term) | CATTGCAGGAATTCAACACG | ATAACCGTTTCTCGTAAGAAT |
| es1 NM_131039.1 | TCACAACACCCGTGAACCTT | GGTCTCCCACATGAAGGTCG |
| gnat1 NM_131868.2 | AGTGCATACGACATGGTGCT | GATGTGGTGGCGAAGTAACG |