Figure 7.

Cluster of SPG3A HSP-causative mutations proximal to the N416 insertion.A, localization of an apparent mutational hotspot demarcated on the structure of ATL1 WT in the prehydrolysis, engaged state (PDB 3Q5E). HSP-associated, mutated residues shown in green and colored by atom on the right. Asterisk (∗) indicates R416, which directly precedes the insertion mutation described here. Black boxes indicate boundaries of zoom-in view on the right in (A and B). B, localization of the cluster in the structure of the tight crossover dimer bound to GDP•AlF₄⁻ (PDB 4IDO; (25)). Coloring as in (A) for chain B and chain A is shown in gray.