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NXT1-mediated enhancement of RevM10-TAP function occurs at the level of RNA export. Northern blot analysis of cytoplasmic (A) and nuclear (B) RNAs is shown. 293T cells were transfected with the indicated constructs. Nuclear or cytoplasmic poly(A)-selected RNA was isolated and analyzed. Blots were hybridized with probes complementary to HIV Gag (Gag/Pol) or SEAP (SEAP) coding sequences.
