Table 4.
List of Media Compositions Used in Cholangiocyte Organoid Cultures in This Study
| Components | Concentration | Source |
|---|---|---|
| N2 | 1% | Gibco (Amarillo, TX) |
| B27 | 1% | Gibco |
| N-acetylcysteine | 1.25 mmol/L | Sigma-Aldrich |
| Gastrin | 10 nmol/L | Sigma-Aldrich |
| Epidermal growth factor | 50 ng/mL | Peprotech |
| Fibroblast growth factor 10 | 100 ng/mL | Peprotech |
| Hepatocyte growth factor | 25 ng/mL | Peprotech |
| R-spondin | 10% | Conditioned medium |
| Nicotinamide | 10 nmol/L | Sigma-Aldrich |
| A83.01 | 5 μmol/L | Tocris |
| Forskolin | 10 μmol/L | Tocris |
| Noggin | 25 ng/mL | Conditioned medium |
| Wnt | 30% | Conditioned medium |
| Y27632 | 10 μmol/L | Sigma-Aldrich |
| hES cell cloning recovery supplement | 2 μmol/L | Stemgent |