Table 2.
Oligonucleotide sequences of primers used for Q-PCR
| Primer | Forward | Reverse |
|---|---|---|
| Murine CNNE1 | 5’-CCCTGGGATGATAATTCAGC-3’ | 5’-TCTGGGTGGTCTGATTTTCC-3’ |
| Murine CNNE2 | 5’-TCTGTGCATTCTAGCCATCG-3’ | 5’-GTCATCCCATTCCAAACCTG-3’ |
| Murine C-MYC | 5’-CTGTGGAGAAGAGGCAAACC-3’ | 5’-TTGTGCTGGTGAGTGGAGAC-3’ |
| Human CXCR4 | 5’-TCCATTCCTTTGCCTCTTTTGC-3’ | 5’-ACGGAAACAGGGTTCCTTCAT-3’ |
| Murine CXCR4 | 5’-TGGAACCGATCAGTGTGAGT-3’ | 5’-TTGCCGACTATGCCAGTCAA-3’ |
| Murine Cyp2e1 | 5’-TGGGGAAACAGGGTAATGAG-3’ | 5’-GTGCACAGCCAATCAGAAAG-3’ |
| Human EPO | 5’-GAGCCCAGAAGGAAGCCATC-3’ | 5’-GCGGAAAGTGTCAGCAGTGA-3’ |
| Murine EPO | 5’-CAGCCACCAGAGACCCTTC-3’ | 5’-ACATCAATTCCTTCTGAGCTCCC-3’ |
| Murine FLK1 | 5’-GGCGGTGGTGACAGTATCTT-3’ | 5’-GTCACTGACAGAGGCGATGA-3’ |
| Murine F4/80 | 5’-GTACAGATGGGGGATGACCAC-3’ | 5’-GACTGAGTTAGGACCACAAGGTGAG-3’ |
| Human GAPDH | 5’-TGGTATCGTGGAAGGACTCATGAC-3’ | 5’-ATGCCAGTGAGCTTCCCGTTCAGC-3’ |
| Murine HIF-1α | 5’-TCAAGTTCAGCAACGTGGAAG-3’ | 5’-TATCGAGGCTGTGTCGACTG-3’ |
| Murine HIF-2α | 5’-AGAGCTGAGGAAGGAGAAATC-3’ | 5’-ATGTGTCCGAAGGAAGCTG-3’ |
| Murine IRF-4 | 5’-GCAGCTCACTTTGGATGACA-3’ | 5’-CCAAACGTCACAGGACATTG-3’ |
| Murine Ki67 | 5’-CATGCAAACCCTCACACTTG-3’ | 5’-GCTGGTTCCAATTTCTGAGC-3’ |
| Murine matrix metalloprotease 9 | 5’-CGTCGTGATCCCCACTTACT-3’ | 5’-AACACACAGGGTTTGCCTTC-3’ |
| Murine PCNA | 5’-CTGTGCAAAGAATGGGGTGAA-3’ | 5’-AGCAAACGTTAGGTGAACAGG-3’ |
| Murine PD-L1 | 5’-AATGCTGCCCTTCAGATCAC-3’ | 5’-TCAGCGTGATTCGCTTGTAG-3’ |
| Murine SB3 | 5’-TTTTACACAAGTCCTTTGTGGAGG-3’ | 5’-CTGGACACATGGAAGAGACACCAC-3’ |
| Murine TBP | 5’-CACATCACAGCTCCCCACCA-3’ | 5’-AGCGGAGAAGATGCTGGAAAC-3’ |
| Murine VE-cadherin | 5’-ATTGAGACAGACCCCAAACG-3’ | 5’-TTCTGGTTTTCTGGCAGCTT-3’ |
| Murine VEGF-A | 5’-CAGGCTGCTGTAACGATGAA-3’ | 5’-TTTCTTGCGCTTTCGTTTTT-3’ |
| Murine YAP | 5′-TCAGACAACAACATGGCAGGA-3′ | 5′-TTCATGGCTGAAGCCGAGTT-3′ |
| Murine αSMA | 5’-CTGACAGAGGCACCACTGAA-3’ | 5’-CATCTCCAGAGTCCAGCACA-3’ |
αSMA, alfa smooth muscle actin; EPO, erythropoietin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IRF-4, interferon regulatory factor - 4; PD-L1, programmed death-ligand 1; TBP, TATA box binding protein; VE, vascular endothelial; VEGF, vascular endothelial growth factor.