Table 2.
Overview of samples used for next generation sequencing analysis.
| No. | CSF volume (μl) | DNA yield (ng/μl) | No. of total reads | Passed QC1 (%) | No. of reads2 | Viruses detected3 |
|---|---|---|---|---|---|---|
| 1 | 150 | 0.048813 | 2,741,706 | 75.98 | 1,640,976 | · Propionibacterium virus ATCC29399BC (442 reads) · Pepino mosaic virus (116 reads) |
| 2 | 250 | 0.053985 | 2,135,708 | 66.87 | 574,390 | · Carrot cryptic virus (14 reads) |
| 3 | 250 | 0.047843 | 2,778,556 | 72.05 | 871,482 | · Pa6virus (550 reads) · Streptococcus phage IPP16 (76 reads) · Spodoptera frugiperda granulovirus (2 reads) |
| 4 | 75 | 0.049244 | 1,458,200 | 55.37 | 336,931 | – |
| 5 | 75 | 0.048705 | 2,244,666 | 65.29 | 534,895 | · Acinetobacter phage Acj61 (214 reads) |
| 6 | 200 | 0.049136 | 2,189,290 | 75.29 | 584,093 | · Acinetobacter phage Acj61 (210 reads) |
1
passed quality control (QC) percentage represents the proportion of reads that passed sequence quality thresholds;
2
the number of original sequencing reads that are sent to downstream analysis after host and quality filtering;
3
only reads with an alignment length ≥30bp are shown.