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. 2021 Dec 7;9:656625. doi: 10.3389/fcell.2021.656625

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Copyright © 2021 Wang, Li, Wang, Zheng, An, Liu, Wang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of cytoplasmic and nuclear p65 proteins in response to hypoxia. (A,B) The expression levels of the cytoplasmic and nuclear p65 proteins in the hPASMCs upon hypoxia treatment (72 h) with and without the transfections of pcDNA-p65 and shp65. Each experiment was repeated three times. The differences were analyzed using one-way ANOVA. CTRL, control. (C) The immunofluorescence assay of the cytoplasmic p65 protein in the hPASMCs upon the above treatments.