Figure 6.

Imiquimod induces the upregulation of multiple anti-viral gene expression pathways in human bronchial epithelial cells (HBECs) exposed to the viral replication mimic poly(I:C). (A) Volcano plot showing differentially expressed genes between poly(I:C) + imiquimod treated versus poly(I:C)-stimulated HBECs, N = 3. Multiple t-test analyses with the corrected method of Benjamini and Yekutieli were used. An adjusted p-value < 0.05 was considered significant. (B) Heat map representation of the 25 most down-regulated and up-regulated genes in response to imiquimod treatment. The most relevant overrepresented Gene Ontology (GO) terms are depicted. N = 3. SIGIRR (C) and C1QBP (D) mRNA expression in HBECs from asthmatic subject (N = 7) in response to poly(I:C) or in combination with imiquimod. Wilcoxon matched-pairs signed rank test for comparisons between two different treatment conditions. *p < 0.05.