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. 2021 Sep 29;8:256–264. doi: 10.1016/j.aninu.2021.06.012

Fig. 2.

Fig. 2

Effect of dietary lycopene supplementation on muscle fiber type conversion in longissimus dorsi (LD) muscle of finishing pigs. (A) Western blotting measured the protein expression levels of slow myosin heavy chain (MyHC) and fast MyHC. (B) Western blotting measured the protein expression level of myoglobin. (C) Immunofluorescence analyzed the percentage of slow-twitch fiber and fast-twitch fiber. The white scale bars represent 100 μm. (D) Real-time quantitative PCR analyzed the mRNA levels of MyHC I, MyHC IIa, MyHC IIx, MyHC IIb and troponin I type 1 (TNNI1). (E to G) The activities of succinic dehydrogenase (SDH), malate dehydrogenase (MDH) and lactate dehydrogenase (LDH) enzymes. (H) The mRNA levels of mitochondrial biogenesis related genes including cytochrome c (Cytc), nuclear respiratory factor 1 (NRF1), mitochondrial transcription factor A (TFAM), mitochondrial transcription factor B1 (TFB1M), citrate synthase (CS) and cyclooxygenase 1 (COX1). (I) The protein level of Cytc. Data are presented as the mean and SEM (n = 6). a, b, c Bar with a different letter indicates significant difference (P < 0.05).