Table 2.

Primer sequences used in this study.

Gene	Primer	Sequence (5′ to 3′)	GenBank ID	Size, bp
MyHC I	Forward	CTTCTACAGGCCTGGGCTTAC	NM_080728	128
	Reverse	CTCCTTCTCAGACTTCCGCAG
MyHC IIa	Forward	TTCCAGAAGCCTAAGGTGGTC	NM_001039545	94
	Reverse	GCCAGCCAGTGATGTTGTAAT
MyHC IIx	Forward	CAACCCATACGACTACGCCT	NM_030679	119
	Reverse	CATCAGAAGTGAAGCCCAGAAT
MyHC IIb	Forward	CTTGTCTGACTCAAGCCTGCC	NM_010855	158
	Reverse	TCGCTCCTTTTCAGACTTCCG
TNNI1	Forward	TGAAGCCAAATGCCTCCACAACAC	NM_006529382	155
	Reverse	ACACCTTGTGCTTAGAGCCCAGTA
AMPKα1	Forward	CGGCAAAGTGAAGGTTGG	NM_001167633	123
	Reverse	AGGTTCTGAATTTCTCTGCGG
AMPKα2	Forward	TGAGGTCGATATCTGGAGCTG	NM_214266	151
	Reverse	AGTGGCAACAGAACGATTGAG
Sirt1	Forward	ACAGTGACAGTGGCACATGC	NM_019812	130
	Reverse	AATCCAGATCCTCCAGCACA
PGC-1α	Forward	CCAGTACAACAATGAGCCTGC	NM_008904	118
	Reverse	CAATCCGTCTTCATCCACG
Cytc	Forward	TGCGGAGTGTTAAACTTTTCAGG	NM_001129970	191
	Reverse	TGCCTTAACAGGCTAGTGAACA
NRF1	Forward	CCTTGTGGTGGGAGGAATGTT	XM_005657993	77
	Reverse	AGTATGCTGGCTGACCTTGTG
TFAM	Forward	GCTCTCCGTTCAGTTTTGCG	AY923074	187
	Reverse	GGAAGTTCCCTCCACAGCTC
TFB1M	Forward	GCAAGCAGTGAAGCAGCTA	NM_001128475	82
	Reverse	CAGACTGCCAGCTTTCCTTAC
CS	Forward	AGCCCTCAACAGTGAAAGCA	NM_026444	174
	Reverse	TCAATGGCTCCGATACTGCTG
COX1	Forward	GTGAGTCAGTCACCTTGAGC	NM_001025218	180
	Reverse	TCTGGCCTACTCAGGAAGGA
SOD1	Forward	AGACCTGGGCAATGTGACTG	NM_001190422	102
	Reverse	GTGCGGCCAATGATGGAATG
SOD2	Forward	TGAACAACCTGAACGTCGTG	NM_214127.2	102
	Reverse	AGCGGTCAACTTCTCCTTGA
CAT	Forward	CAGATGAAGCATTGGAAGGAGC	NM_214301	83
	Reverse	TTGTCTCCTATCGGATTCCCAG
GPX1	Forward	GTGAATGGCGCAAATGCTCA	NM_214201	126
	Reverse	ATTGCGACACACTGGAGACC
GST	Forward	CCAACCCAGAAGACTGCTCA	AB000884	102
	Reverse	CATTCAGGTGGGCTCTTCGT
GR	Forward	GTGAGCCGACTGAACACCAT	XM_003483635	102
	Reverse	CAGGATGTGAGGAGCTGTGT
Nrf2	Forward	GCCCCTGGAAGCGTTAAAC	XM_003133500	67
	Reverse	GGACTGTATCCCCAGAAGGTTGT
Keap1	Forward	ACGACGTGGAGACAGAAACGT	NM_001114671	56
	Reverse	GCTTCGCCGATGCTTCA
GAPDH	Forward	ACTCACTCTTCTACCTTTGATGCT	NM_001206359	100
	Reverse	TGTTGCTGTAGCCAAATTCA

MyHC I = myosin heavy chain I; MyHC IIa = myosin heavy chain IIa; MyHC IIx = myosin heavy chain IIx; MyHCIIb = myosin heavy chain IIb; TNNI1 = troponin I type 1; AMPKα1 = AMP-activated protein kinase α1; AMPKα2 = AMP-activated protein kinase α2; Sirt1 = sirtuin1; PGC-1α = peroxisome proliferator activated receptor-γ coactivator-1α; Cytc = cytochrome c; NRF1 = nuclear respiratory factor 1; TFAM = mitochondrial transcription factor A; TFB1M = mitochondrial transcription factor B1; CS = citrate synthase; COX1 = cyclooxygenase 1; SOD1 = superoxide dismutase 1; SOD2 = superoxide dismutase 2; CAT = catalase; GPX1 = glutathione peroxidase 1; GST = glutathione S-transferase; GR = glutathione reductase; NRF2 = nuclear factor erythroid 2-related factor 2; Keap1 = Kelch-like ECH-associated protein 1; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.