Table 1.
Ingredients and chemical composition of experimental diets of phase 1 (%, as-fed basis).1
| Item | PC | NC | NC1 | NC2 | NC3 | NC4 |
|---|---|---|---|---|---|---|
| Ingredients | ||||||
| Corn | 57.08 | 61.44 | 61.28 | 61.08 | 60.92 | 60.72 |
| Soybean meal | 36.66 | 31.34 | 31.36 | 31.4 | 31.42 | 31.46 |
| Animal fat | 2.31 | 2.6 | 2.66 | 2.73 | 2.79 | 2.86 |
| Di-calcium phosphate | 1.65 | 1.77 | 1.77 | 1.77 | 1.77 | 1.77 |
| Limestone | 0.84 | 0.81 | 0.81 | 0.81 | 0.81 | 0.81 |
| Salt | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 |
| Met (99%) | 0.18 | 0.21 | 0.21 | 0.21 | 0.21 | 0.21 |
| Lys (78%) | 0.62 | 0.81 | 0.81 | 0.81 | 0.81 | 0.81 |
| Thr (98%) | 0.21 | 0.29 | 0.29 | 0.29 | 0.29 | 0.29 |
| Trp (98%) | 0.02 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| L-Glu (98%) | – | 0.25 | 0.33 | 0.42 | 0.50 | 0.59 |
| Mineral mix2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Vitamin mix3 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Choline (25%) | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 |
| Total | 100 | 100 | 100 | 100 | 100 | 100 |
| Calculated value | ||||||
| ME, kcal/kg | 3,300 | 3,300 | 3,300 | 3,300 | 3,300 | 3,300 |
| CP | 22.69 | 20.69 | 20.69 | 20.69 | 20.69 | 20.69 |
| Ca | 0.85 | 0.85 | 0.85 | 0.85 | 0.85 | 0.85 |
| P | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 |
| Lys | 1.7 | 1.7 | 1.7 | 1.7 | 1.7 | 1.7 |
| Met | 0.51 | 0.51 | 0.51 | 0.51 | 0.51 | 0.51 |
| Thr | 1.06 | 1.05 | 1.05 | 1.05 | 1.05 | 1.05 |
| Trp | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 |
| Glu | 4 | 3.83 | 3.91 | 4 | 4.08 | 4.17 |
| Crude fat | 4.81 | 5.16 | 5.22 | 5.28 | 5.33 | 5.4 |
| Crude fiber | 2.72 | 2.61 | 2.61 | 2.61 | 2.61 | 2.6 |
| Ash | 4.57 | 4.57 | 4.57 | 4.57 | 4.57 | 4.57 |
| Analyzed value | ||||||
| CP | 22.7 | 20.67 | 20.72 | 20.7 | 20.65 | 20.73 |
| Ca | 0.83 | 0.84 | 0.84 | 0.85 | 0.85 | 0.85 |
| P | 0.69 | 0.69 | 0.7 | 0.68 | 0.71 | 0.71 |
| Lys | 1.68 | 1.68 | 1.68 | 1.69 | 1.71 | 1.71 |
| Met | 0.5 | 0.5 | 0.49 | 0.5 | 0.49 | 0.51 |
| Thr | 1.07 | 1.05 | 1.05 | 1.03 | 1.03 | 1.04 |
| Trp | 0.32 | 0.31 | 0.28 | 0.28 | 0.28 | 0.29 |
| Glu | 4.00 | 3.85 | 3.90 | 3.99 | 4.10 | 4.17 |
| Crude fat | 4.8 | 5.15 | 5.22 | 5.27 | 5.32 | 5.4 |
| Crude fiber | 2.73 | 2.63 | 2.61 | 2.61 | 2.59 | 2.6 |
| Ash | 4.57 | 4.55 | 4.55 | 4.58 | 4.59 | 4.56 |
PC (positive control), the basal standard protein diet with the Lys-to-Glu ratio at 1:2.35; NC (negative control), NC1, NC2, NC3, and NC4, the basal diet with 2% CP reduction and Lys-to-Glu ratios at 1:2.25, 1:2.30, 1:2.35, 1:2.40, and 1:2.45, respectively.
Provided per kilogram diet: Fe, 180 mg as ferrous sulfate; Cu, 17 mg as copper sulfate; Mn, 54 mg as manganese oxide; Zn, 90 mg as zinc oxide; I, 0.78 mg as potassium iodide; and Se, 0.36 mg as sodium selenite.
Provided per kilogram diet: vitamin A, 10,800 IU; vitamin D3, 4,000 IU; vitamin E, 40 IU; vitamin K3, 4 mg; vitamin B1, 6 mg; vitamin B2, 21.6 mg; vitamin B6, 9.6 mg; vitamin B12, 0.084 mg; biotin, 0.48 mg; folic acid, 3 mg; niacin, 84 mg; D-calcium pantothenate, 54 mg.