Table 2.
Ingredients and chemical composition of experimental diets of phase 2 (%, as-fed basis). 1
| Item | PC | NC | NC1 | NC2 | NC3 | NC4 |
|---|---|---|---|---|---|---|
| Ingredients | ||||||
| Corn | 62.77 | 67.12 | 66.92 | 66.77 | 66.62 | 66.45 |
| Soybean meal | 31.32 | 26 | 26.04 | 26.06 | 26.08 | 26.11 |
| Animal fat | 2.18 | 2.48 | 2.55 | 2.6 | 2.66 | 2.72 |
| Di-calcium phosphate | 1.5 | 1.65 | 1.66 | 1.66 | 1.66 | 1.66 |
| Limestone | 0.85 | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 |
| Salt | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 |
| Met (99%) | 0.15 | 0.18 | 0.18 | 0.18 | 0.18 | 0.18 |
| Lys (78%) | 0.59 | 0.78 | 0.78 | 0.78 | 0.78 | 0.78 |
| Thr (98%) | 0.19 | 0.28 | 0.28 | 0.28 | 0.28 | 0.28 |
| Trp (98%) | 0.02 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| L-Glu (98%) | – | 0.23 | 0.31 | 0.39 | 0.46 | 0.54 |
| Mineral mix2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Vitamin mix3 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Choline (25%) | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 |
| Total | 100 | 100 | 100 | 100 | 100 | 100 |
| Calculated value | ||||||
| ME, kcal/kg | 3,300 | 3,300 | 3,300 | 3,300 | 3,300 | 3,300 |
| CP | 20.56 | 18.56 | 18.56 | 18.56 | 18.56 | 18.56 |
| Ca | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 |
| P | 0.65 | 0.65 | 0.65 | 0.65 | 0.65 | 0.65 |
| Lys | 1.53 | 1.53 | 1.53 | 1.53 | 1.53 | 1.53 |
| Met | 0.44 | 0.44 | 0.44 | 0.44 | 0.44 | 0.44 |
| Thr | 0.95 | 0.95 | 0.95 | 0.95 | 0.95 | 0.95 |
| Trp | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Glu | 3.6 | 3.44 | 3.52 | 3.6 | 3.67 | 3.75 |
| Crude fat | 4.8 | 5.16 | 5.22 | 5.27 | 5.32 | 5.38 |
| Crude fiber | 2.64 | 2.54 | 2.53 | 2.53 | 2.53 | 2.52 |
| Ash | 4.57 | 4.57 | 4.57 | 4.57 | 4.57 | 4.57 |
| Analyzed value | ||||||
| CP | 20.59 | 18.6 | 18.55 | 18.54 | 18.52 | 18.58 |
| Ca | 0.78 | 0.82 | 0.82 | 0.82 | 0.8 | 0.82 |
| P | 0.64 | 0.66 | 0.63 | 0.63 | 0.63 | 0.66 |
| Lys | 1.55 | 1.53 | 1.53 | 1.51 | 1.51 | 1.52 |
| Met | 0.42 | 0.44 | 0.42 | 0.43 | 0.43 | 0.44 |
| Thr | 0.94 | 0.95 | 0.97 | 0.97 | 0.96 | 0.96 |
| Trp | 0.23 | 0.27 | 0.24 | 0.24 | 0.27 | 0.25 |
| Glu | 3.62 | 3.42 | 3.54 | 3.61 | 3.69 | 3.73 |
| Crude fat | 4.79 | 5.16 | 5.23 | 5.27 | 5.33 | 5.39 |
| Crude fiber | 2.65 | 2.53 | 2.55 | 2.51 | 2.53 | 2.54 |
| Ash | 4.56 | 4.59 | 4.59 | 4.56 | 4.56 | 4.55 |
PC (positive control), the basal standard protein diet with the Lys-to-Glu ratio at 1:2.35; NC (negative control), NC1, NC2, NC3, and NC4, the basal diet with 2% CP reduction and Lys-to-Glu ratios at 1:2.25, 1:2.30, 1:2.35, 1:2.40, and 1:2.45, respectively.
Provided per kilogram diet: Fe, 180 mg as ferrous sulfate; Cu, 17 mg as copper sulfate; Mn, 54 mg as manganese oxide; Zn, 90 mg as zinc oxide; I, 0.78 mg as potassium iodide; and Se, 0.36 mg as sodium selenite.
Provided per kilogram diet: vitamin A, 10,800 IU; vitamin D3, 4,000 IU; vitamin E, 40 IU; vitamin K3, 4 mg; vitamin B1, 6 mg; vitamin B2, 21.6 mg; vitamin B6, 9.6 mg; vitamin B12, 0.084 mg; biotin, 0.48 mg; folic acid, 3 mg; niacin, 84 mg; D-calcium pantothenate, 54 mg.