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. 2021 Dec 21;106(1):401–414. doi: 10.1007/s00253-021-11701-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Immune reactivity of IpaB and IpaH-CTD produced in CFPS. Serum IgG titers against IpaB (a) or IpaH-CTD (b) in 14 volunteers pre- and post-challenge with S.flexneri 2a (circles) and 5 volunteers pre- and post-immunization with live-attenuated S.flexneri 2a (squares) measured by ELISA using IpaB or IpaH-CTD produced in CFPS versus titers obtained by ELISA using IpaB produced in E. coli (a) or versus IpaH-specific antibody binding signals obtained in a protein microarray (b). Pearson correlation coefficient (r) and p value are indicated