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. 2021 Dec 7;12:771300. doi: 10.3389/fpls.2021.771300

Figure 2.

Figure 2

Functional characterization of HpDGAT1 in Saccharomyces cerevisiae cells. (A) TLC and Nile red staining analysis of total lipids extracted from control S. cerevisiae (INVSc1), TAG-deficient S. cerevisiae (H1246), and H1246 cells transformed with HpDGAT1 and Empty vector (EV). (B) The transcriptional expression levels of HpDGAT1 in H1246 and INVSc1 cells transformed with HpDGAT1. The gene expression levels were normalized to the endogenous ScACTIN gene. (C) TAG contents and relative abundance of fatty acids in INVSc1 and H1246 cells transformed with HpDGAT1. (D) Relative abundance of fatty acids in H1246 cells transformed with HpDGAT1 by the feeding of free fatty acids of C18:2n6, C18:3n3, C18:3n6, and C20:4n6, respectively, after a 24 h cultivation. (E) The in vitro substrate specificities of HpDGAT1 for acyl CoAs. C1, microsome plus eukaryotic DAG; C2, microsome plus prokaryotic DAG; and C3, microsome. (F) Relative abundance of fatty acids in INVSc1 cells by the feeding of free fatty acids of C18:2n6, C18:3n3, C18:3n6, and C20:4n6, respectively, after 24 h cultivation.