Figure 3.

Effects of B355227 on glutamate-induced oxidative stress in HT22 cells. Control cells and cells treated with glutamate in the presence or absence of B355227 were assessed for levels of GSH, ROS and Ca²⁺. (A) Measurement of GSH level. Cells co-treated with B355227 demonstrated a significant increase in the levels of GSH. (B) Evaluation of intracellular ROS. Glutamate-induced increase in intracellular ROS was suppressed following treatment with B355227. (C) Measurement of intracellular Ca²⁺. Glutamate-induced increase in intracellular Ca²⁺ was suppressed following treatment with B355227. Data are presented as the mean (n=3) ± standard deviation obtained from three independent experiments. ^*P<0.05 vs. control group; ^#P<0.05 vs. glutamate treated group. GSH, glutathione; ROS, reactive oxygen species.