Figure 4.

Effects of B355227 on the glutamate-dependent collapse of the MMP. The integrity of the MMP was determined in HT22 cells treated with glutamate in the presence or absence of B355227. Confocal images demonstrating the collapse of the MMP induced by glutamate are represented by a decrease in cellular fluorescence (center left) and protection of the MMP by B355227 is represented by markedly increased fluorescence (center right). Magnification, x40. Bars represent densitometric quantification of the corresponding images. The data are presented as the mean (n=3) ± standard deviation obtained from three independent experiments. ^*P<0.05 vs. control group; ^#P<0.05 vs. glutamate treated group. MMP, mitochondrial membrane potential.