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. 2021 Dec 7;12:795010. doi: 10.3389/fgene.2021.795010

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Copyright © 2021 Tan, Fenner, Barathi, Tun, Wey, Tsai, Su, Lee, Cheung, Wong, Mehta and Teo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fundus autofluorescence images of non-human primate eyes demonstrating the improved efficacy of retinal gene transfection after surgical removal of the internal limiting membrane (ILM). Both eyes in the figure had intravitreal delivery of AAV2 vector carrying the gene for green fluorescent protein (GFP) after a pars plana vitrectomy. One eye (A) had surgical peeling of the ILM over the macula prior to exposure to the AAV2 vector, while the other eye (B) did not. The eye that underwent ILM removal (A) shows significant hyper-autofluorescence over the macular region from GFP expression, while the other does not (B).