Figure 1.

The armed oncolytic adenovirus expressing decorin (OAV-Decorin) successfully inhibits renal cancer cell (RCC) growth in vitro

(A) Schematic representation of genome of the oncolytic adenoviruses used in this study. (B) ACHN, 786O, and OSRC-2 cells were infected with OAV-DEC at the indicated MOIs; 48 h later, the key adenoviral E1A protein was confirmed by Western blot. GAPDH was used as a loading control. (C, D) Supernatants were obtained 48 h post infection by OAV-DEC (referred to OAV-Decorin), and the concentration of decorin and TGF-β were analyzed by ELISA assay. (E) ACHN, 786O, and OSRC-2 cells were infected with OAV-DEC at a various MOIs (1, 5, 20, and 50) for 48, 72, or 96 h, respectively. Cell viability was measured using the CCK-8 assay. Relative cell viability was calculated compared with the control group, which was set at 1. (F) ACHN, 786O, and OSRC-2 cells were infected with OAV-DEC at various MOIs (5, 20, and 50). Viable cells were calculated as “cell index” using the xCELLigence RTCA system. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.