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. 2021 Dec 7;8:777370. doi: 10.3389/fmolb.2021.777370

FIGURE 2.

FIGURE 2

PD-1H expression in tumor-infiltrating immune cells assessed by multiplex immunohistochemistry (mIHC). (A) Representative images of mIHC staining of PD-1H, CD4, CD8, CD68, and DAPI in ESCC tumor tissues. Scale bar: 25 μm, original magnification × 400. (B) The cell numbers of PD-1H+CD68+ myeloid cells were significantly higher than cell numbers of PD-1H+CD4+ and PD-1H+CD8+ cells. (C) The percentage of PD-1H positive cells among CD4+ T cells, CD8+ T cells, and CD68+ cells was estimated. (D) The expression score of PD-1H was calculated and classified into high-expression (PD-1H high) or low-expression (PD-1H low) groups by using the R package of “survMisc”. (E) The absolute counts of immune cells and CD4+ T cells in tumor sites were significantly higher in patients with high PD-1H expression than in patients with low PD-1H expression. ns = non-significant, *p < 0.05, **p < 0.01.