Conventional Bactec MGIT (MGIT tube containing PANTA-OADC antimicrobial enrichment media) and TiKa (MGIT tube containing PANTA-OADC and TiKa supplement B) culture results represented as median time to positivity (d) with interquartile ranges (IQR) for sterile phosphate buffer spiked in duplicate with known amounts (cfu) of A.
Mycobacterium bovis SB0121 (10,000–10 cfu) and B.
M. tuberculosis H37Rv (10,000–10 cfu), respectively. Prior to inoculation into growth indicator tubes, all spiked samples were either: decontaminated with MycoPrep (treatment 1: MycoPrep MGIT; treatment 2: MycoPrep TiKa), not decontaminated (treatment 3: MGIT control; treatment 4: TiKa control), or decontaminated with KiC agent (treatment 5: KiC MGIT; treatment 6: KiC TiKa). For some data points, the IQRs were shorter than the height of the bar; in these cases, the ranges were not drawn. Using the multiple t-test with Bonferroni–Dunn correction for multiple testing (alpha = 0.01), statistical significance (p < 0.01) was calculated between treatments for each amount of mycobacteria. Within each amount of the target Mycobacterium, bars with a different superscript are significantly different at p < 0.01.