TABLE 4.
Phytochemicals | Phytochemical tests | Observation | (Pet. ether) | (EtOAc) | (EtOH) |
---|---|---|---|---|---|
Proteins | 1) Ninhydrin test | Blue/violet color/ppt | +++ | ++ | − |
2) Biuret test | +++ | ++ | + | ||
Carbohydrates | 1) Fehling’s test | Brick red ppt | + | ++ | +++ |
2) Benedict’s test | + | ++ | +++ | ||
Alkaloids | Wagner’s test | Reddish-brown | + | + | ++ |
Saponins | Foam test | Foam on surface | +++ | + | − |
Phenols and tannins | Ferric chloride test | Bluish-green | + | + | + |
Terpenoids and steroids | Salkowski test | Reddish-brown | ++ | + | +++ |
Glycosides | A) Keller–Kilani test | Brown ring/reddish-brown | ++ | + | +++ |
B) Salkowski test | ++ | + | +++ | ||
Anthocyanins | Hydrochloric acid test | Blue color | ++ | + | + |
Quinones | Sulfuric acid test | Red color | + | + | + |
Flavonoids and coumarins | Sodium hydroxide test | Yellow color | + | + | ++ |
+Slightly present, ++ present, +++ strongly present, − absent.