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. 2021 Dec 7;12:774583. doi: 10.3389/fphar.2021.774583

TABLE 4.

Preliminary phytochemical screening of extracts of Ficus benghalensis.

Phytochemicals Phytochemical tests Observation (Pet. ether) (EtOAc) (EtOH)
Proteins 1) Ninhydrin test Blue/violet color/ppt +++ ++
2) Biuret test +++ ++ +
Carbohydrates 1) Fehling’s test Brick red ppt + ++ +++
2) Benedict’s test + ++ +++
Alkaloids Wagner’s test Reddish-brown + + ++
Saponins Foam test Foam on surface +++ +
Phenols and tannins Ferric chloride test Bluish-green + + +
Terpenoids and steroids Salkowski test Reddish-brown ++ + +++
Glycosides A) Keller–Kilani test Brown ring/reddish-brown ++ + +++
B) Salkowski test ++ + +++
Anthocyanins Hydrochloric acid test Blue color ++ + +
Quinones Sulfuric acid test Red color + + +
Flavonoids and coumarins Sodium hydroxide test Yellow color + + ++

+Slightly present, ++ present, +++ strongly present, − absent.