REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-B220-PE Ab	Biolegend	Cat#103208 RRID; AB_312993
Anti-BST2-FITC Ab	Biolegend	Cat#127008 RRID; AB_2028462
Anti-Bcl6-PE Ab	eBioscience Thermo Fisher Scientific	Cat#12-5760-80 RRID; AB_10852866
Anti-CD3 Ab	Biolegend	Cat#100302 RRID; AB_312667
Anti-CD3-PE/Cy7 Ab	Biolegend	Cat#100320 RRID; AB_312685
Anti-CD4 Ab	Biolegend	Cat#100402 RRID; AB_312687
Anti-human CD4-PE Cy7 Ab	Biolegend	Cat#300511 RRID; AB_314079
Anti-mouse CD4-FITC Ab	Biolegend	Cat#100510 RRID; AB_312913
Anti-CD4-PerCP/Cy5.5 Ab	Biolegend	Cat#100540 RRID; AB_893326
Anti-CD5-FITC Ab	Biolegend	Cat#100605 RRID; AB_312734
Anti-CD11c-APC Ab	BD Biosciences	Cat#550261 RRID; AB_398460
Anti-CD16/32 Ab	Biolegend	Cat#101302 RRID; AB_312801
Anti-CD21-FITC Ab	Biolegend	Cat#123407 RRID; AB_940403
Anti-CD23-PE Ab	Biolegend	Cat#101607 RRID; AB_312832
Anti-CD23-Alexa Fluor 647 Ab	Biolegend	Cat#101611 RRID; AB_493479
Anti-CD25-PE Ab	Biolegend	Cat#10200 RRID; AB_312857
Anti-CD27-FITC Ab	Biolegend	Cat#124207 RRID; AB_1236463
Anti-CD28 mAb	Biolegend	Cat#102102 RRID; AB_312867
Anti-CD43 activation-associated glycoform-APC Ab	Biolegend	Cat#121213 RRID; AB_528806
Anti-CD44-FITC Ab	Biolegend	Cat#103005 RRID; AB_312956
Anti-human CD45RB-Alexa Fluor 488 Ab	eBioscience Thermo Fisher Scientific	Cat#53-9458-82 RRID; AB_2574448
Anti-CD62L-APC Ab	Biolegend	Cat#104411313098 RRID; AB_
Anti-CD80-FITC Ab	Biolegend	Cat#104705 RRID; AB_313126
Anti-CD83-FITC Ab	Biolegend	Cat#121505 RRID; AB_572008
Anti-CD86-PE Ab	Biolegend	Cat#105008 RRID; AB_313157
Anti-human CD122-APC Ab	Biolegend	Cat#339007 RRID; AB_2248891
Anti-CD123-PE Ab	Biolegend	Cat#106005 RRID; AB_2124403
Anti-CD138-PE Ab	Biolegend	Cat#142503 RRID; AB_10915989
Anti-CD147-FITC Ab	Biolegend	Cat#123705 RRID; AB_1227513
Anti-CCR4-PE/Cy7 Ab	Biolegend	Cat#131213 RRID; AB_2074506
Anti-CXCR5-FITC Ab	Biolegend	Cat#145520 RRID; AB_25622866
Anti-CXCR5-APC Ab	Biolegend	Cat#145506 RRID; AB_2561970
Anti-CD19-PE/Cy7 Ab	Biolegend	Cat#115519 RRID; AB_313654
Anti-DOCK8 rabbit Ab	Proteintech	Cat#11622-1-AP RRID; AB_10216360
Au-goat anti-rabbit IgG H&L Ab	Abcam	Cat#ab27234 RRID; AB_954427
Anti-fluorescein-ALP Ab	Amersham Pharmacia	Cat#RPN3690 RRID; AB_
Anti-Foxp3-APC Ab	eBioscience	Cat#14-5773-80 RRID; AB_467575
Anti-FR4-FITC Ab	Biolegend	Cat#125005 RRID; AB_1134204
Anti-GATA3-Alexa Fluor 488 Ab	eBioscience	Cat#53-9966-41 RRID; AB_2574492
Anti- GL7-FITC Ab	Biolegend	Cat#144603 RRID; AB_2561696
Anti-GL7-PE Ab	Biolegend	Cat#144607 RRID; AB_2562295
Anti-ICOS-FITC Ab	Biolegend	Cat#313505 RRID; AB_416329
Anti-acetyl Histone H3 Ab	Millipore Sigma	Cat#06-599 RRID; AB_2115283
Anti-trimethyl Histone H3 (Lys4) Ab	Millipore Sigma	Cat#07-473RRID; AB_1977252
Anti-Histone H3 (trimethyl K4) Ab. ChIP grade	Abcam	Cat#ab8580 RRID; AB_306649
Anti-rabbit IgG-PE Ab	Biolegend	Cat#406421 RRID; AB_2563484
Anti-rabbit IgG-FITC Ab	Biolegend	Cat#406403 RRID; AB_893531
Anti-rabbit IgG-HRP Ab	Cappel Laboratories	Cat#55641
Anti-mouse IgG-Cy3 Ab	Biolegend	Cat#405309 RRID; AB_893530
Anti-human IgG Fc-PE Ab	Biolegend	Cat#409304 RRID;AB_10895907
Anti-IgM-FITC Ab	Biolegend	Cat#406505 RRID; AB_315055
Anti-IFNγ rabbit Ab	Abcam	Cat#ab96575 RRID; AB_306649
Anti-LFA1-APC Ab	Biolegend	Cat#141009 RRID; AB_2564305
Anti-Ly6C-FITC Ab	BD Pharmingen	Cat#561085 RRID; AB_10584332
Anti-PD-1-APC Ab	Biolegend	Cat#135210 RRID; AB_2159183
Anti-RORγt-APC Ab	eBioscience Thermo Fisher Scientific	Cat#17-6981-82 RRID; AB_2573254
Anti-Siglec H-PE Ab	Biolegend	Cat#129605 RRID; AB_1227763
Anti-T-bet-Alexa Fluor 647 Ab	Biolegend	Cat#644803 RRID; AB_15955573
Anti-Thy1.2-APC Ab	Biolegend	Cat#105311 RRID; AB_313182
Anti-TCRβ-APC Ab	Biolegend	Cat#109211 RRID; AB_313434
Anti-TLR7 Ab	BD Biosciences	Cat#565557 RRID; AB_2739295
Anti-Vβ8 TCR-PE Ab	BD Biosciences	Cat#553862 RRID; AB_395098
Chemicals, peptides, and recombinant proteins
Alpha-galactosylceramide (αGalCer)	Kirin Brewery Co.	Gift
Alkaline phosphatase avidin D	Vector Laboratories	Cat#A-2100
Animal-free blocker	Vector Laboratories	Cat#SP5030
AccuCheck counting beads	Life Technologies	Cat#PCB100
Brefeldin A	Sigma-Aldrich	Cat#B7651
Calf thyms DNA	Worthington Biochem	Cat#DNA
CFSE	Thermo Fisher Scientific	Cat#C34570
CpG	Invitrogen	Cat#tlrl-2395
Dynabeads Protein G	VERITAS	Cat#DB10003
E.coli DNA polymerase	Invitrogen	Cat#18010017
E.coli DNA ligase	Invitrogen	Cat#18052019
Extraction buffer I & II	EMB Bioscience	Cat#539790
Epon 812	TAAB	Cat#T023
FCS (fetal calf serum)	BioWest	Cat#S1820-500
Fast red	Nichirei Bioscience	Cat#415261
16-pad FAST slides	EMD Milipore	Cat#Z721115-20EA
Histogreen	LINARIS	Cat#E109
Hybond N+ membrane	GE Healthcare	Cat#RPN1210B
Ionomycin	Sigma-Aldrich	Cat#10634
Immobilon-P membrane	Milipore	Cat#IPVH00010
KAPA HiFi DNA polymerase	Kapa Biosystems	Cat#KK2101
KLH (keyhole limpet hemocyanin)	Sigma-Aldrich	Cat#H7017
Lead staining solution	Sigma-Aldrich	Cat#18-0875-2-25ML-J
LPS (lipopolysaccharide)	Sigma-Aldrich	Cat#L4516
Anti-rabbit IgG MicroBeads	Miltenyi Biotech	Cat#130-048-602
Mouse CD4 MicroBeads	Miltenyi Biotech	Cat#130-049-201
Nonidet P-40 (NP-40)	MP Biochemicals	Cat#198595
Nylon membrane	Roche Diagnostic	Cat#1 417 240 001
Normal rabbit IgG	Invitrogen	Cat#10500C
Nonimmunized rabbit IgG	Cappel Laboratories	Cat#55944
Ovalbumin (OVA)	Sigma-Aldrich	Cat#A5503
OsO4	Nisshin EM	Cat#300-1
PMA (phorbol myristate acetate)	Sigma-Aldrich	Cat#P1585
Protein G magnetic beads	Invitrogen	Cat#100-03D
Peanut agglutinin (PNA)-fluorescein	Vector Laboratories	Cat#FL-1071
Polyacrylamide gel	Sigma-Aldrich	Cat#GF90890466
Protease inhibitor cocktail	EMD Milipore	Cat#539790
Proteinase K	TaKaRa Bio Inc.	Cat#9033
RNase A	Qiagen	Cat#19101
RNase H	Invitrogen	Cat#18021071
Sph I restriction enzyme	TaKaRa Bio Inc.	Cat#1246
Superscript III reverse transcriptase	Invitrogen	Cat#18081044
Saponin	Sigma-Aldrich	Cat#S4521
Sm antigen	ImmunoVision	Cat#SMA-3000
SEB (staphylococcus enterotoxin B)	Toxin Technologies	Cat#BT202
SEA (staphylococcus enterotoxin A)	Toxin Technologies	Cat#TA101
T4 DNA polymerase	Invitrogen	Cat#18005025
T4 DNA ligase	TaKaRa Bio Inc.	Cat#2011A
T4 DNA ligase	Roche Diagnostic	Cat#481220
Tween 20	FUJIFILM Wako	Cat#167-11515
Uranyl acetate	EM Sciences	Cat#22400
Critical commercial assays
Mouse IFNγ ELISA kit	Biolegend	Cat#430804
Mouse Il-2 ELISA kit	Biolegend	Cat#431004
Mouse IL-4 ELISA kit	Biolegend	Cat#431104
Mouse IL-6 ELISA kit	Biolegend	Cat#431304
Mouse IL-10 ELISA kit	Biolegend	Cat#431414
Mouse IL-17 ELISA kit	Biolegend	Cat#432504
Mouse IL21 ELISA kit	eBioscience Thermo Fisher Scientific	Cat#88-8210-22 RRID 2575209
Mouse IL-22 ELISA kit	eBioscience Thermo Fisher Scientific	Cat#88-7422-22; RRID 2572119
Mouse IL-23 ELISA kit	Biolegend	Cat#433707
Mouse TGFβ ELISA kit	eBioscience Thermo Fisher Scientific	Cat#88-8350-22; RRID 2575209
Mouse TNFα ELISA kit	eBioscience Thermo Fisher Scientific	Cat#88-7324-22; RRID 2575076
Biotin labelling kit-HN2	Dojindo Molecular Technologies	Cat#LK03
Cell Trace CFSE cell proliferation kit	Thermo Fisher Scientific	Cat#C34554
CDP-Star chemiluminiscence	Amersham Pharmacia	Cat#RPN3690
Dynabeads M280-Tosylactivated	Thermo Fisher Scientific	Cat#14203
Expi 293 system	Thermo Fisher Scientific	Cat#A14635
Foxp3/transcription factor staining buffer set	eBioscience	Cat#00-5523-00
MACS Beads mouse CD4 isolation kit	Miltenyi Biotech	Cat#130-104-454
MinElute Reaction Cleanup kit	Qiagen	Cat#28206
MaxiSorp immunotubes	Thermo Fisher Scientific	Cat#444474
Nextera XT Index kit v2	Illumine	Cat#FC-131-1002
ProteoExtract Subcellular Proteome Extraction kit	EMD Milipore	Cat#539790
Peroxidase detection system	Leica Biosystems	Cat#RE7110-k
RF ELISA kit	FUJIFILM Wako	Cat#AKRRG-101
RNeasy Mini kit	Qiagen	Cat#74106
SurePrint G3 mouse GE Microassay	Agilent Tchnology	Cat#8x60K ver2.0
QIA quick PCR purification kit	Qiagen	Cat#28104
Deposited data
Supplemental information; ShunichiShiozawa (2021), “TCRA and TCRB repertoire analysis Results”, Mendeley Data, V1, doi:https://doi.org/10.17632/7r5pjbz793.1	This paper, Mendeley Data	https://data.mendeley.com//datasets/7r5pjbz793/2
Gene expression microarray analysis Results of DOCK8+CD4 T cells, DOCK8-CD4 T cells of 12x OVA-immunized mice and CD4 T cells of 12x PBS-immunized mice	This paper, NCBI’s Gene Expression Omnibus	GEO Series accession number GSE159240.
Oligonucleotides
Primer and probe sequences used in CHIP assay	This paper	Table S3