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. 2021 Dec 7;12:690371. doi: 10.3389/fphar.2021.690371

FIGURE 5.

FIGURE 5

NaHS attenuated the Warburg effect and ERS in HL-1 cells under Ang II treatment. Representative Western blotting and relative densitometry analysis of (A) CSE with GAPDH as a loading control induced by Ang-II concentration in the HL-1 cell of the indicated groups (n = 6 in each group). (B) Detection of CSE activity by methylene blue assay in the HL-1 cell of the indicated groups (n = 6 in each group). CCK-8 was used to detect the changes in HL-1 cell viability induced by Ang II at different times (C) (n = 6 in each group). Representative Western blotting and relative densitometry analysis of (F) p-PERK with t-PERK as the loading control and (H) p-elF2α with t-elF2α as the loading control and (D) LDH, (E) PDK-4, (G) ATF 4, (I) CHOP and (J) caspase12 with GAPDH as the loading control in the HL-1 cell of the indicated groups (n = 6 in each group). Lactate acid (K) and glucose consumption (L) and the production of ATP (M) were determined by kit analysis in the HL-1 cell of the indicated groups (n = 6 in each group). **p < 0.01 VS Control, and p < 0.01 VS Ang II. Groups were compared using one-way analysis of variance (ANOVA) (Tukey’s) in (A–J).