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. 2021 Dec 7;12:690371. doi: 10.3389/fphar.2021.690371

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Copyright © 2021 Hu, Wang, Liu, Zhang, Chen, Zhang, Tang, Qu, Tang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NaHS inhibits HL-1 oxidative stress induced by Ang II and is associated with attenuation of the Warburg effect and ERS. (A) Red staining with representative flow cytometry and quantification of the HL-1 cell apoptotic rate (B) were used as indication treatments (n = 6 in each group). (C) CCK-8 was used to detect the changes in HL-1 cell viability induced by the indicated treatments (n = 6 in each group). After different treatments, ROS were measured using DCFH-DA staining followed by photofluorography (n = 6 in each group, scale bar = 50 μm) and quantitative analysis of the mean fluorescence intensity (MFI) in each group. (D) Control, (E) Ang II, (F) Ang II + NaHS, (G) Ang II + DCA, (H) Ang II + 4-PBA, (I) NaHS, (J) DCA and (K) 4-PBA. 8-OHdG (L), lipid peroxides (M) and Carbonyl protein (N) were determined by kit analysis in the HL-1 cell of the indicated groups (n = 6 in each group). Mitochondrial oxygen consumption was measured using a Clark-type oxygen electrode to detect (O) The respiratory control rate (RCR), (P) The ADP/O ratio, (Q) The mitochondrial state3 oxygen consumption, (R) The mitochondrial state4 oxygen consumption in the HL-1 cell of the indicated groups (n = 6 in each group)**p < 0.01 VS Control, *p < 0.05 VS Control, and p < 0.05 VS Ang II, #p < 0.01 VS Ang II. Groups were compared using one-way analysis of variance (ANOVA) (Tukey’s) in (D–R), Fisher’s exact test was used to compare groups in (B,C).