REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti FasII mouse monoclonal	DSHB	Monoclonal 1D4
Anti Acetylated alpha tubulin (K40) mouse monoclonal	protein tech	Cat# 66200
Anti Hsc 70 K-19 goat polyclonal	Santa Cruz	Cat# sc-1059
Anti Dmel Atx2 rabbit polyclonal	Li Antibodies custom preparation	N/A
Anti alpha tubulin DM1a mouse monoclonal	Prepared in house	N/A
Anti tubulin rb polyclonal	Prepared in house	N/A
HRP-conjugated anti mouse	Jackson	RRID: AB_2340770
HRP-conjugated anti rabbit	Jackson	RRID: AB_10015282
HRP-conjugated anti goat	Jackson	RRID: AB_2340390
Alexafluor 647 conjugated anti mouse IgG	Jackson	RRID: AB_2340862
Chemicals, peptides, and recombinant proteins
Latrunculin B	Sigma Aldrich	Cat#L5288 CAS number 76343-94-7
Vinblastine	Sigma Aldrich	Cat#V1377 CAS number 143-67-9
Rhodamine conjugated Phalloidin	Thermo Fisher Scientific	Cat#R415
Effectene	Qiagen	Cat#301425
Critical commercial assays
TruSeq stranded mRNA kit	Illumina	Cat#20020596
Deposited data
RNA seq data	This paper	GSE185473
Experimental models: Cell lines
S2R+	DGRC	RRID:CVCL_A5UM
Experimental models: Organisms/strains
Drosophila melanogaster Ataxin-2 RNAi TRiP line #1 (Val20, second chromosome attP40 insertion targeting CDS 561-581) TRiP.HMS02726	Bloomington Stock Centre (BDSC)	RRID:BDSC_44102
Drosophila melanogaster Ataxin-2 RNAi TRiP line #2 (Val20, third chromosome attP2 insertion targeting Atx2 CDS 474-494) TRiP.HMS01392	Bloomington Stock Centre (BDSC)	RRID:BDSC_36114
Drosophila melanogaster elavP-gal4	Bloomington Stock Centre (BDSC)	RRID:BDSC_8760
Drosophila melanogaster ppk-CD4-tdTomato	Bloomington Stock Centre (BDSC)	RRID:BDSC_35844
Drosophila melanogaster UASt-mCherry.mito.OMM	Bloomington Stock Centre (BDSC)	RRID:BDSC_66532
Drosophila melanogaster Ubi-EB1-GFP	S. Rogers (Shimada et al., 2006)
Drosophila melanogaster D42 gal4>UAS ANF GFP	A. Lim, W. Saxton (Lim et al., 2017)
Drosophila melanogaster Nrv2-GFP	Yale FlyTrap database	ZCL2903
Software and algorithms
Fiji	http://fiji.sc	Version 2.0.0-rc-69/1.52p
Graphpad Prism	https://www.graphpad.com/scientific-software/prism/	Version 9.1.0 (216)
Nikon Elements	https://www.nikon.com/products/industrial-metrology/support/download/software/imgsfw/	Version 4.00.07
PANTHER GO	http://www.pantherdb.org	Version 16.0
Recombinant DNA
pMT-EOS-alpha-Tubulin	This lab (Barlan et al., 2013).