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. 2021 Dec 7;9:731810. doi: 10.3389/fcell.2021.731810

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Copyright © 2021 Dong, Yu, Shan, Su, Yu and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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METTL3 was highly expressed in ox-LDL-treated HUVECs. (A–D) HUVECs were treated with different concentrations of ox-LDL (0, 1, 5, 10, or 20 μg/ml). (A) Cell proliferative activity was examined through CCK-8 assay at 12 h or 24 h after ox-LDL exposure. (B) Cell migratory ability was analyzed using Transwell migration assay at 24 h after ox-LDL treatment. (C) Tube formation capacity was assessed by tube formation assay at 12 h after ox-LDL stimulation. (D) The global m6A level in total RNA was measured using a commercial kit at 24 h after ox-LDL stimulation. (E) At 24 h after ox-LDL treatment, mRNA levels of METTL3, METTL14, WTAP, VIRMA, FTO, and ALKBH5 were measured through RT-qPCR assay. Three independent experiments were performed for all in vitro assays. *p < 0.05, **p < 0.01, ***p < 0.001.