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. 2021 Dec 7;9:731810. doi: 10.3389/fcell.2021.731810

FIGURE 5.

FIGURE 5

METTL3 exerted its functions by regulating JAK2 expression and JAK2/STAT3 pathway. (A) HUVECs were transfected with pcDNA-JAK2 plasmid or empty vector. Forty-eight hours later, JAK2 mRNA level was measured by RT-qPCR assay. (B–F) HUVECs were co-transfected with si-NC + pcDNA3.1, si-METTL3+pcDNA3.1, or si-METTL3+pcDNA-JAK2 for 24 h and then stimulated with ox-LDL. At 24 h after ox-LDL treatment, JAK2, p-STAT3, and STAT3 protein levels (B), cell proliferative (C) and migratory (D) capacities, and VEGF secretion level (F) were measured. (E) At 12 h after ox-LDL treatment, tube formation patterns were measured. Three independent experiments were performed for all in vitro assays. **p < 0.01, ***p < 0.001.