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. 2021 Nov 24;298(1):101448. doi: 10.1016/j.jbc.2021.101448

Figure 10.

Figure 10

USP10-KD reduces the amount of phosphorylated p62.A, SH-SY5Y cells were transfected with USP10-siRNA (siUSP10-2) or control (siNT) using Lipofectamine RNAiMAX. Cells were treated with 0.4 mM dopamine or DMSO for 8 h. Cell lysates prepared from transfected cells were immunoprecipitated with anti-p62 antibody or normal rabbit IgG. Cell lysates (input) and immunoprecipitates (IP) were characterized by Western blotting using the indicated antibodies. B, SH-SY5Y cells were transfected with USP10-siRNA (siUSP10-2) or control (siNT) using Lipofectamine RNAiMAX. The cells were pretreated with 5 nM BafA1 30 min before dopamine treatment and further treated with 0.4 mM dopamine or DMSO for 8 h. Cell lysates were characterized by Western blotting using the indicated antibodies. USP10, ubiquitin-specific protease 10.