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. 2021 Nov 24;298(1):101448. doi: 10.1016/j.jbc.2021.101448

Figure 6.

Figure 6

Dopamine enhances PERK phosphorylation.A, SH-SY5Y cells were pretreated with 1 μM GSK260614 (PERK inhibitor) or DMSO 30 min before dopamine treatment and then treated with 0.2 to 0.4 mM dopamine or DMSO for 8 h. In addition, cells were treated with 1 μM thapsigargin (Tg) for 30 min, and this was used as positive control for PERK phosphorylation. Whole cell lysates prepared from these cells were characterized by Western blotting using the indicated antibodies. B, SH-SY5Y cells were transfected with USP10-siRNA (USP10-1 or 2) or control (NT) using Lipofectamine RNAiMAX. Cells were treated with 0.4 mM dopamine or DMSO for 4 and 8 h. Whole cell lysates prepared from transfected cells were characterized by Western blotting using the indicated antibodies. USP10, ubiquitin-specific protease 10.