Figure 9.

USP10-KD induces ubiquitination of ribosomal proteins. SH-SY5Y cells were transfected with USP10-siRNA (USP10-1 or 2) or control (NT) using Lipofectamine RNAiMAX. Cells were treated with 0.4 mM dopamine or DMSO for 4 and 8 h. Whole cell lysates prepared from transfected cells were characterized by Western blotting using the indicated antibodies. The experiments in Figures 6B and 9 were performed simultaneously as one experiment. Therefore, the data for β-actin and USP10 shown in Figure 6B are also represented in Figure 9. USP10, ubiquitin-specific protease 10.