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. 2021 Nov 22;55:101401. doi: 10.1016/j.molmet.2021.101401

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

The effect of DREADD activation of PVN^MC4R+neurons on feeding and glucose homeostasis. (A) Schematic showing bilateral microinjection of Cre-dependent AAV driving expression of excitatory hM3Dq-DREADD receptor into the PVN of MC4R-Cre⁺ mouse. (B) Representative images showing mCherry and c-Fos immunoreactivity in the PVN of MC4R-Cre⁺ mice that received either AAV-DIO-mCherry or AAV-DIO-hM3Dq-mCherry (Scale bar: 100 μm). (C) Quantification of c-Fos⁺ PVN mCherry cells. (D) Decreased food pellets consumption (FED3) after IP injection of CNO at the beginning of dark cycle. (E) The effects of DREADD activation of PVN^MC4R+ neurons on GTT (n = 7 for mCherry and n = 8 for hM3Dq). ∗∗∗p < 0.001 by Student's t-test (C and pellets consumed 5 h post CNO in D) or by two-way ANOVA (time course pellets consumption in D). Data are presented as mean ± SEM.