Figure 2.

Cryo-EM sample preparation in practice

(A) Liquid nitrogen cools down ethane in the middle metal container using a spider-like piece for heat conduction in a designed foam container. The blue button is for grid storage.

(B) The grids are glow discharged.

(C) The grid is loaded on the tweezer and lifted into the MarkIV vitrobot chamber, the liquid ethane container is lifted to the bottom of the chamber.

(D) Load the sample from one side of the chamber onto the grid, blot the grid with filter papers.

(E) Plunge the grid into liquid ethane and transfer to the blue grid button for storage.