Lysis Buffer

Reagent	Final concentration	Amount
Tris-HCl pH 7.5 (1 M)	20 mM	2 mL
NaCl (5 M)	1 M	20 mL
Imidazole (5 M)	20 mM	0.4 mL
2-mercaptoethanol (BME) (14.3 M)	5 mM	0.35 mL
ddH2O	n/a	77.25 mL
Total	n/a	100 mL

Filter and sterilize with a 0.22 μm filter. The lysis buffer should be made fresh and pre-cooled at 4°C before use. The lysis buffer is recommended to store no more than a week to avoid contamination.