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PBS
| Reagent | Final concentration | Amount |
|---|---|---|
| Na2HPO4 (0.2 M) | 10 mM | 50 mL |
| KH2PO4 (0.2 M) | 2 mM | 10 mL |
| NaCl (5 M) | 137 mM | 27.4 mL |
| KCl (0.2 M) | 2.7 mM | 13.5 mL |
| ddH2O | n/a | 899.1 mL |
| Total | n/a | 1,000 mL |
Add concentrated hydrochloric acid to adjust the pH to 7.4, and then add deionized water to make the solution volume to 1,000 mL. After autoclaving, The buffer can be stored at room temperature for no more than a week to avoid contamination.
