SDS-PAGE loading buffer

Reagent	Final concentration	Amount
Tris-HCl pH 6.8 (1 M)	250 mM	1.25 mL
Sodium dodecyl sulfate (SDS)	10% (W/V)	0.50 g
Bromophenol blue (BPB)	0.5% (W/V)	0.025 g
Glycerol	50% (V/V)	2.50 mL
2-mercaptoethanol (BME) (14.3 M)	5% (V/V)	0.25 mL
ddH2O	n/a	to 5 mL
Total	n/a	5 mL

Store at room temperature for about a month.