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5×SDS-PAGE running buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris | 0.125 M | 15.1 g |
| Glycine | 1.25 M | 94 g |
| SDS | 0.5% (W/V) | 5 g |
| ddH2O | n/a | to 1000 mL |
| Total | n/a | 1000 mL |
Before use, dilute with ddH2O to 1×SDS-PAGE running buffer. Store at room temperature for about a month.
