Storage buffer

Reagent	Final concentration	Amount
Tris-HCl pH 7.5 (1 M)	20 mM	2 mL
NaCl (5 M)	500 mM	10 mL
Tris(2-carboxyethyl)phosphine (TCEP) (1 M)	2 mM	0.2 mL
ddH2O	n/a	87.8 mL
Total	n/a	100 mL

Filter and sterilize with a 0.22 μm filter. Store at room temperature for about a week.