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MS horizontal plates containing MS nutrients, 1% sucrose, pH 5.7–5.8. Store MS plates at 4°C for up to 1 week.
| Reagent | Final concentration | Amount |
|---|---|---|
| Murashige and Skoog (MS) Basal Medium | n/a | 4.34 g |
| Agar | 0.5% | 5 g |
| Sucrose | 1% | 10 g |
| ddH2O | n/a | Up to 1 L |
| Total | n/a | 1 L |
Adjust the MS nutrients pH to 5.7–5.8 with 0.5 M KOH, and autoclave at 110°C for at least 30 min. Cool to ∼60°C and pour into a disposable Petri dish after autoclave.
