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Innate lymphoid cell (ILC) panel
| Fluorophore | Marker | Clone | Final dilution |
|---|---|---|---|
| PerPCy5.5 | CD3ε | 145-2C11 | 1/200 |
| CD5 | 53-7.3 | 1/200 | |
| CD19 | 6D5 | 1/200 | |
| CD11b | M1/70 | 1/200 | |
| CD11c | N418 | 1/200 | |
| FcεRIα | MAR-1 | 1/200 | |
| NK1.1 | PK136 | 1/200 | |
| CD2 | RM2-5 | 1/200 | |
| BV421 | CD45 | 30F-11 | 1/100 |
| BV711 | MHC ll | M5/114.15.2 | 1/3000 |
| APC-Cy7 | Sca-1 | D7 | 1/200 |
| BUV395 | Thy1.2 | 53-2.1 | 1/200 |
| PE-Cy7 | CCR6 | 29-2L17 | 1/200 |
To detect transcription factors (GATA3 and RORγt), staining is performed after cell surface staining (Table 5).
